XDY-1 inverted fluorescence microscope is composed with EPI-fluorescence microscope and inverted microscope, equipped long working distance plan achromatic objectives and wide field eyepieces. The enable turning out or into condenser system working distance is longer and suited for observation in a high culture dish, can be used in phase-contrast microscopy. The Epi-fluorescence microscope system is adopted modularization function design idea, so that adjust the fluorescence illuminating system and switching-over fluorescence filters safely and quickly. This is a sort of ideal optical instrument for micro observation in cell tissue and transmitted liquid tissue, even in dynamic observation in the culture dish tissue, can be applied in the fluorescence microscopy, such as biological pharmacy, medicine checking and measure, disease prevent and etc.